Inm-2: New Approaches in Cryopreservation

Authors

  • Hassani F
Abstract:

Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have been successfully treated with high-dose chemotherapy or radiotherapy. Although many young patients receiving these treatments are at risk of developing reproductive failure, a number of fertility preservation options ranging from embryo cryopreservation to ovarian tissue cryopreservation are now available. Embryo cryopreservation is a well established technique for preserving fertility. The success rate with oocyte cryopreservation has been on the rise. Both oocyte and embryo freezing require ovarian stimulation and novel ovarian stimulation regimens utilizing aromatase inhibitors which have been developed for ovarian stimulation in women with estrogen sensitive cancer. Although ovarian tissue cryopreservation is a novel technology, it is the only fertility preservation option for children and the only treatment strategy that can restore ovarian function. In vitro maturation is a promising technology and can be applied in conjunction with ovarian tissue cryopreservation. There are 2 main methods for transplantation of frozen- thawed ovarian tissues: orthotopic and heterotopic transplantation. These technologies are investigational, although rapidly evolving, and their list of appropriate indications may expand in the future.

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Journal title

volume 6  issue 2

pages  -

publication date 2012-09-01

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